Journal: International Journal of Molecular Sciences

Article Title: Green Synthesized Titanium Oxide Nanoparticles Promote Salt Tolerance in Soybean

doi: 10.3390/ijms26178309

Figure Lengend Snippet: Immunoblot analysis of proteins involved in soybean treated with TiO 2 NPs under salt stress. Six treatments were performed: control, CS-TiO 2 NPs, GS-TiO 2 NPs, salt, CS-TiO 2 NPs + salt, and GS-TiO 2 NPs + salt. Proteins extracted from root and hypocotyl of soybean were separated on SDS-polyacrylamide gel by electrophoresis. Proteins were transferred onto PVDF membranes. The membranes were cross-reacted with anti-superoxide dismutase, peroxiredoxin, ascorbate peroxidase, and glutathione reductase antibodies. The integrated densities of the bands were calculated using ImageJ software. Data analysis and statistical calculations are the same as in (**, p < 0.01; *, p < 0.05).

Article Snippet: After blocking, the PVDF membrane was cross-reacted with the primary antibodies for 30 min. As the primary antibodies, anti-V ATPase (Agrisera, Vännäs, Sweden), ascorbate peroxidases [ ], glutathione reductase (Agrisera), Cu/Zn superoxide dismutase (Proteintech, Rosemont, IL, USA), and peroxiredoxin [ ] antibodies were used.

Techniques: Western Blot, Control, Polyacrylamide Gel Electrophoresis, Software